Family 2.A.12 - The ATP:ADP Antiporter Family       

Family ID: 52620

Members of the AAA family have been sequenced from bacteria and plants. One protein from the obligate intracellular bacterial parasite, Rickettsia prowazekii, the etiologic agent of the human disease epidemic typhus, is of 498 amino acyl residues and is believed to span the membrane 12 times. The transporter is an obligate exchange translocase specific for ATP and ADP. It functions to take up ATP from the eukaryotic cell cytoplasm into the bacterium in exchange for ADP. The bacteria thus gains energy in the form of one pyrophosphate bond per ATP molecule taken up. Five AAA family paralogues are encoded within the genome of R. prowazekii. This organism transports UMP and GMP but not CMP, and it seems likely that one or more of the AAA family paralogues are responsible.

The AAA family proteins are not related to the mitochondrial ATP/ADP exchangers of the Mitochondrial Carrier Family (MCF; TC #2.A.5) which pump ATP out of mitochondria in accordance with the polarity of the mitochondrial membrane potential. However, two homologous adenylate translocators of the plant, Arabidopsis thaliana, have been sequenced and characterized. They are reported to be 589 and 569 amino acyl residues in length, possess twelve putative transmembrane spanners, and are about 85% identical to each other. They are about 44% identical to the rickettsial translocator described above. They are postulated to be localized to the intracellular plastid membrane where they function as ATP importers.